The emergence of antibiotic-resistant bacteria such as MRSA (Methicillin-Resistant Staphylococcus aureus) is due to bacterial mutations and the use of antibiotic drugs that are not by following with procedures. Resistance makes MRSA infections difficult to treat, resulting in high healthcare costs. MRSA can be transmitted by direct contact or through contaminated objects. This problem causes an urgent need to find alternatives to controlling MRSA infection, one of which is using Moringa oleifera plant-based nanoparticle antibacterial cloth. Moringa oleifera contains several active compounds such as alkaloids, flavonoids, and saponins which are known to have antibiotic activity. Silver nanoparticles or AgNPs are currently used as antimicrobial agents because they are toxic to prokaryotic cells (bacteria) but relatively safe for eukaryotic cells. AgNP synthesis mediated by M. oleifera extract has the advantages of being non-toxic, pollution-free, and environmentally friendly. Bioactive chemicals from plant-extracted nanoparticles also provide additional antimicrobial properties. This study aims to produce AgNPs mediated by M. oleifera leaf extract and to analyze their antimicrobial effect on MRSA growth. The biologically active compounds were extracted from the leaves of M. oleifera by the Soxhlet extraction method with ethyl-acetate extraction solvent (sample ratio: 1:10 solvent). The synthesis of nanoparticles from AgNO3 was carried out with the help of M. oleifera leaf extract. The highest content of silver nanoparticles (based on UV-Vis absorbance) was obtained by mixing 10 mM AgNO3 and 10 mg/ml M. oleifera leaf extract for 24 hours with stirring at 140 rpm. The resulting AgNPs had a MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bacteriocidal Concentration) value of 1.25 mg/ml. The resulting silver nanoparticles showed antibiotic activity against MRSA with an average inhibition zone diameter of 15.677 mm.